Thyroid hormone actions are temperature-specific and regulate thermal acclimation in zebrafish (Danio rerio)

Table 6 Two-way ANOVA results comparing parameters between cold-acclimated normal thryoid, hypothyroid, and T ₂ - or T ₃ -supplemented fish ^a

	Thyroid status			Test temperature			Thyroid status × test temperature
	F	d.f.	P	F	d.f.	P	F	d.f.	P
U_crit ^b	12.689	3, 65	0.001**	31.085	1, 65	0.001**	0.959	3, 65	0.417
RMR	1.466	3, 55	0.234	4.253	1, 55	0.044*	0.209	3, 55	0.890
AMR	3.535	3, 55	0.020*	11.032	1, 55	0.002**	0.050	3, 55	0.985
Scope	3.170	3, 55	0.006**	50.558	1, 55	0.005**	0.132	3, 55	0.899
LDH^c	11.025	3, 64	0.001**	130.539	1, 64	0.001**	0.516	3, 64	0.673
COX^c	1.421	3, 48	0.248	24.904	1, 48	0.001**	0.192	3, 55	0.902

Abbreviations: AMR, active metabolic rate; COX, cytochrome c oxidase; d.f., degrees of freedom; MS, metabolic scope; LDH, lactate dehydrogenase; RMR, resting metabolic rate.
^aCitrate synthase was not measured because there was no significant effect of thyroid hormone in the cold-acclimated and warm-acclimated normal thyroid and hypothyroid fish.
^bSwimming performance.
^cMaximal activity.
*P<0.05.
**P<0.01.

ISSN: 1741-7007