Enhanced detection of 5mC through Tet1 oxidation using in vitro methylated plasmid samples. The Circos plots show the plasmid-wide view of IPD ratios for (a) untreated and (b) mouse Tet1-treated samples, with the outer and inner circle denoting the forward and reverse DNA strands, respectively, and the blue tick marks denoting all positions of the targeted M.HpaII in vitro methylated sequence motif of 5'-CCGG-3' (methylated base underlined). The bracket denotes a section of the plasmid which is shown at base resolution in the bottom graphs, containing three instances of the methylated motif (grey boxes). The methylated positions are highlighted in red. IPD: interpulse duration.