Enhanced 5-methylcytosine detection in single-molecule, real-time sequencing via Tet1 oxidation

Table 1 Detection of 5mC in native versus mTet1-enhanced SMRT sequencing for the bacterial genomes

Sample		Methylation motif	Number in genome	Number detected	Number detected (%)	Number unassigned (%)^a
E. coli	native	C^mCWGG	24,079	455	1.9	0.4
MG1655	Tet1	C^mCWGG	24,079	22,913	95.2	0.3
B. halodurans	native	GG^mCC	15,207	660	4.3	0.6
C-125	Tet1	GG^mCC	15,207	11,663	76.7	0.5

^a Unassigned is the percentage of genomic positions that have kinetic scores above the cutoff but are not in a methylated motif or a secondary peak.

ISSN: 1741-7007