Representative micrographs showing distribution of TUNEL-positive cells (green) in the uninjured and regenerating RNC. The radial glial cells are stained with the ERG1 monoclonal antibody (magenta), and the nuclei are stained with DAPI (blue). All micrographs are longitudinal sections with the plane of injury or growing regenerate to the left. (A) Uninjured RNC. A’ and A” show a high magnification view of the TUNEL-positive ERG1-negative cell marked with an arrowhead. (B) Abundant TUNEL-positive cells in the vicinity of the injury plane at the early post-injury stage (day 1). (C) Late post-injury stage (day 8). (D) Growing glial tubular rudiment on day 10 post-injury. D’ and D” show a high magnification view of a TUNEL-positive ERG1-positive glial cell (marked with an arrow). (E) Late regenerate (day 21 post-injury).