Table 1 Antibodies used in the present study
From: Radial glial cells play a key role in echinoderm neural regeneration
Antibodies used | Source | Host species | Dilutions |
|---|---|---|---|
AFRU | Dr. J. M. Grondona (University of Malaga, Spain) | Rabbit | 1:1000 – 1:4000 |
BrdU | GenWay (20-783-71418) | Rat | 1:400 |
ERG1 | Mashanov et al. [22] | Mouse | 1:1 |
GABA | Sigma (A2052) | Rabbit | 1:1000 – 1:2000 |
GFSKLYFamide | Diaz-Miranda et al. [31] | Rabbit | 1:500 – 1:1000 |
Nurr1/Nur77 (E-20) | Santa Cruz Biotechnology (SC-990) | Rabbit | 1:250 – 1:1000 |
AMCA-conjugated AffiniPure Goat Anti-Rat IgG (H+L) | Jackson ImmunoResearch Laboratories, Inc. (112-155-003) | Goat | 1:50 |
Cy3-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) | Jackson ImmunoResearch Laboratories, Inc. (112-165-146) | Goat | 1:2000 |
Cy3-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) | Jackson ImmunoResearch Laboratories, Inc. (112-165-144) | Goat | 1:2000 |
FITC-conjugated Goat Anti-Mouse IgG | Biosource (AMI0408) | Goat | 1:100 |
FITC-conjugated Goat Anti-Rabbit IgG | Biosource (ALI0408) | Goat | 1:100 |
FITC-conjugated Goat Anti-Rat IgG (H+L) | GenWay (25-787-278232) | Goat | 1:50 |