PmLox and PmCdx relative expression domains in the sea star developing gut. (A, B, C) Double in situ hybridization of PmLox (green) and PmCdx (magenta) expression domains coupled with nuclei staining (blue, obtained with DAPI) in sea star embryo and larvae. In A, a gastrula embryo is shown in frontal view. In B and C, larvae are shown in lateral view. Developmental stages are provided in each panel. On the right of each developmental stage, a magnification of the gut domain expressing the two genes is provided, showing first both channels (A’, B’, C’), then the green channel only (A”, B”, C”) and then the magenta panel (A”’, B”’, C”’); nuclear staining is shown in blue. All the pictures represent full projection of confocal z-series. d, days; DAPI, 4',6-diamidino-2-phenylindole.