Figure 5
From: DNA damage strength modulates a bimodal switch of p53 dynamics for cell-fate control
Bimodal switch of p53 dynamics modulates the p53 cytoplasmic localization. (a) Representative single-cell trajectories of average p53 fluorescence in the nucleus (black line) and cytoplasm (red line) under 100 μmol/l etoposide. (b) Western blotting analysis of p53 levels and the indicated p53 post-translational modifications in the nucleus and cytoplasm of U-2 OS cells treated with no drug, 1 μmol/l etoposide (treated for 24 hours) and 100 μmol/l etoposide (treated for 12 hours), respectively. To confirm the quality of the subcellular fractionation, tubulin was used as a cytoplasmic marker and transcriptional factor II B (TFIIB) as a nuclear marker.