The tip of the saccule in E. maculatus is firmly attached to the bone via connective tissue (shown in yellow, B). Three-dimensional reconstructions are based on microCT imaging in (A) and histological serial sections in (B). In (C) the connection is shown in a histological semithin section. sac, saccule. The white arrow in (A) and (B) indicates the attachment site. In (A) cranial bone and cartilage are both shown in red while in (B) bone is transparent red and cartilage transparent dark blue. Scale bars, 250 μm (A-B), 100 μm (C). microCT, microtomography.