Corpora allata knockout of adult Drosophila reduces juvenile hormone. A) Adult corpora allata (yellow cells) marked by Aug21-Gal4 driving UAS-GFP (Aug21, GFP). B) Total JH measured from adults by GC-MS per wet mass (total body). Genotypes with intact corpora allata: Aug21, GFP; UAS-NIPP1, wildtype (WT, [w1118]). Corpora allata knockout (CAKO): Aug21,GFP; UAS-NIPP1. Error bars are standard deviation; significant difference indicated by * (ANOVA, P < 0.000,1).