Adult survival of control and CAKO genotypes, and response to JHA. A) CAKO (in w1118 background) relative to three control genotypes with intact corpora allata (Aug21, GFP; UAS-NIPP1, wildtype (WT(w1118)). B) CAKO (in wDAH background) relative to wildtype (wDAH). C) CAKO (in wDAH/w1118 background) exposed to vehicle (EtOH) relative to CAKO treated with JHA, and compared to wildtype (wDAH/w1118) with EtOH or JHA. D) CAKO (in wDAH/w1118 background) with OvoD1 mutation relative to control wDAH/w1118 with OvoD1 mutation; females of both cohorts produce no eggs. In each panel, CAKO overall mortality is significantly less than each control (log rank test, P < 0.0001) while there are no significant differences among controls within a trial.