Integration-deficient lentivectors: an effective strategy to purify and differentiate human embryonic stem cell-derived hepatic progenitors

Table 1 Primer sequences used for reverse transcription-PCR in this study

Gene	Direction	Primer sequence 5′→3′	Annealing temperature, °C	Amplicon size, bp
FoxM1B	Forward	GGGCGCACGGCGGAAGATGAA	55	492
FoxM1B	Reverse	CCACTCTTCCAAGGGAGGGCTC	55	492
Fibrinogen β	Forward	CAAGGTGTCAACGACAATGAGGAG	55	315
Fibrinogen β	Reverse	CAGGTCTGGGTCAGCGTGAAGAGA	55	315
HNF4α	Forward	CTGCTCGGAGCCACCAAGAGATCCATG	55	370
HNF4α	Reverse	ATCATCTGCCACGTGATGCTCTGCA	55	370
AFP	Forward	AGAACCTGTCACAAGCTGTG	55	675
AFP	Reverse	GACAGCAAGCTGAGGATGTC	55	675
ApoA-II	Forward	GGAGAAGGTCAAGAGCCCGAG	55	246
ApoA-II	Reverse	AGCAAAGAGTGGGTAGGGACAG	55	246
FIX	Forward	GTCCTGTGAACCAGCAGTGCC	56	512
FIX	Reverse	TTGTCAGCAATGCAAATAGGT	56	512
Β-actin	Forward	TCACCACCACGGCCGAGCG	58	350
	Reverse	TCTCCTTCTGCATCCTGTCG

ISSN: 1741-7007