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Table 3 Antibodies used in this study

From: Integration-deficient lentivectors: an effective strategy to purify and differentiate human embryonic stem cell-derived hepatic progenitors

Antibody

Company

Reference number

Dilution

Primary

   

EpCam

AbCys

abc171

1:20

CK19

Dakoa

M0888

1:50

AFP

Dako

N1501

1:300

HNF4α

Santa Cruzb

SC-8987

1:100

E-cadherin

Dako

M3612

1:100

SOX17

R&D

MAB1924

1:100

OCT4

Santa Cruz

SC-5279

1:200

NANOG

R&D

AF1997

1:100

SSEA 4

AbCys

VMA 4304

1:100

TRA 1-60

Millipore

MAB4360

1:100

Secondary

   

Goat anti-mouse

Molecular Probesc

A21123

1:800

Chicken anti-goat

Invitrogen

A21467

1:800

Goat anti-rabbit

Molecular Probes

A11010

1:800

  1. aDako, Glostrup, Denmark.
  2. bSanta Cruz Biotechnology Inc., Santa Cruz, CA, USA.
  3. cMolecular Probes, Eugene, OR, USA.