Table 1 Primers used in quantitative real-time polymerase chain reaction
Primer name | Direction | Sequence |
|---|---|---|
Mouse NDUFA9 (39 kDa) | Sense | 5′-CAT TAC TGC AGA GCC ACT-3′ |
Antisense | 5′-ATC AGA CGA AGG TGC ATG AT-3′ | |
Mouse NDUFB6 (17 kDa) | Sense | 5′-ATA ACT TTT TGC GGG ACG GG-3′ |
Antisense | 5′-CAG GAA AAT CTC TCA TTG GTG-3′ | |
Mouse NDUFS3 (30 kDa) | Sense | 5′-AGG AAC ATG GCG GCG GCT GC-3′ |
Antisense | 5′-ATT TCA GCC ACA TAC TCT CC-3′ | |
Mouse MTND1 (ND1) | Sense | 5′-TGC ACC TAC CCT ATC ACT C-3′ |
Antisense | 5′-ATT GTT TGG GCT ACG GCT C-3′ | |
Mouse MTND2 (ND2) | Sense | 5′-ATG AGT AGG CCT GGA ATT C-3′ |
Antisense | 5′-ATC AGA AGT GGA ATG GGG C-3′ | |
Mouse MTND4 (ND4) | Sense | 5′-ATA ATT ATA ACT AGC TCA ATC TGC-3′ |
Antisense | 5′-TCG TAG TTG GAG TTT GCT AG-3′ | |
Mouse MTND4L (ND4L) | Sense | 5′-CTC ACC ATA GCC TTC TCA C-3′ |
Antisense | 5′-CGT AAT CTG TTC CGT ACG TG-3′ | |
Mouse β-actin | Sense | 5′-ATG GAT GAC GAT ATC GCT G-3′ |
Antisense | 5′-GTT GGT AAC AAT GCC ATG TTC-3′ | |
Mouse UQCRC1 (core 1) | Sense | 5′-CCT ACA GCA CTC GAG AGC AC-3′ |
Antisense | 5′-AGG TGT GCC CTG GAA TGC TG-3′ | |
Mouse UQCRC2 (core 2) | Sense | 5′-TCC CTC AAA GTT GCC CC-3′ |
Antisense | 5′-GCA AGA CGT AGT AAA TGT GAG-3′ | |
Mouse UQCRFS1 (FeS) | Sense | 5′-GAT GTC AAG GTG CCC GAC TT-3′ |
Antisense | 5′-GAT CTC GAT CTT CGA CAT GG-3′ | |
Mouse SDHA (70 kDa) | Sense | 5′-CAT ACT GTT GCA GCA GCA CAG G-3′ |
Antisense | 5′-CCA CCA AAT GCA CGC TGA TA-3′ | |
Mouse COX4 (COX IV) | Sense | 5′-GAG CAC ATG GGA GTG TTG TG-3′ |
Antisense | 5′-CTG TCT TCC ATT CAT TGG TGC C-3′ | |
Mouse PGC1α | Sense | 5′-GAG ACT TTG GAG GCC AGC AAG-3′ |
Antisense | 5′-CCA AGG GTA GCT CAG TTT ATC AG-3′ | |
Mouse ERRα | Sense | 5′-GAC AGT CCA AAG GGT TCC TCA G-3′ |
Antisense | 5′-CTG GAT GGT CCT CTT GAA GAA GGC-3′ | |
Mouse SP1 | Sense | 5′-CTG CCA GCT TGG TGT CAT CAC-3′ |
Antisense | 5′-CTG ACT TCC TTG CAG CGA GC-3′ | |
Human NDUFA9 (39 kDa) | Sense | 5′- GAT TGT GGC CAC TGT GTT TGG-3′ |
Antisense | 5′-CTC CAG CTT CCT TGG ACA GT-3′ | |
Human NDUFS3 (30 kDa) | Sense | 5′-GTC AGA CCA CGG AAT GAT GTG-3′ |
Antisense | 5′-CTC AAA ACG GTT TTG CCG AG-3′ | |
Human NDUFB6 (17 kDa) | Sense | 5′-CTG CAG CAG CTG CGA GA-3′ |
Antisense | 5′-GAA TAA TCC AGA CAG GTA CAA G-3′ | |
Human UQCRC1 (core I) | Sense | 5′-GTT AGC CTG CTG GAC AAC G-3′ |
Antisense | 5′-CTT GAT GTA GTA AGC TGT GTG C-3′ | |
Human UQCRC2 (core II) | Sense | 5′-CCA AAT GGC TTG GTG ATT GC-3′ |
Antisense | 5′-CAT TAG AAT ATC AAC ATC ACC CC-3′ | |
Human UQCRFS1 (FeS) | Sense | 5′-GCC TGT GTT GGA CCT GAA G-3′ |
Antisense | 5′-CTG GAA ACG AAC TGG GTG AC-3′ | |
Human COX4 (COX IV) | Sense | 5′-CAG GGT ATT TAG CCT AGT TGG-3′ |
Antisense | 5′-CTC CTT GAA CTT AAT GCG ATA C-3′ | |
Human SDHA (70 kDa) | Sense | 5′-GGC TTG CGA GCT GCA TTT GG-3′ |
Antisense | 5′-GTT CTG CTA AAC GGC ATG CCA-3′ | |
Human PGC1α | Sense | 5′-GCA AAC TTG CTA GCA GTC CTC-3′ |
Antisense | 5′-GGT ACT GAG CCA ACT GCA TTC-3′ | |
Human ERRα | Sense | 5′-GAG TGT GAG ATC ACC AAG CG-3′ |
Antisense | 5′-CAC ATG AGA CAC CAG TGC ATT C-3′ | |
Human SP1 | Sense | 5′-CAG CAG AAT TGA GTC ACC CAA T-3′ |
Antisense | 5′-CAT ACT GCC CAC CAG AGA C-3′ |