Effective population size ( N
) plotted against predicted estrogen exposure for 37 population samples of Rutilus rutilus . (A) N
calculated using the Approximate Bayesian Computation (ABC) method in the program OneSAMP . Tests for homogeneity of variances: Bartlett’s, P = 0.0036, Levene’s, P = 0.17. (B) Results of binning analysis for data shown in A; each bin which encompasses all data points starting at the lower point represented by the mean and standard error up to, but not including, the next bin. (C) Ne calculated using the sibling assignment (SA) method in Colony . In A and C, error bars are 95% confidence intervals. In cases in which more than one population had similar values, data points overlie each other; thus, individual data points are not always visible. These plots include estimates from sample sites sampled in different years, for example, in the River Nene (N) (which were averaged for statistical analysis) and sites where recent restocking had occurred (open circles: River Aire (A), River Wandle (W)), which were excluded from the statistical analyses.