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Table 1 Sampling locations, genetic diversity statistics (allelic richness ( AR ) and expected heterozygosity ( H e )) for each population sample

From: Populations of a cyprinid fish are self-sustaining despite widespread feminization of males

Sample codea River Year Lat. Long. E2Eq (ng/L)b No. fish genotyped Bottleneck (TPM)c AR H e Ref.d
Blackwater           
BlaBlM Blackwater 2010 51.78529 0.651013 4.1 55 0.40 7.9 0.71  
BlaSti Blackwater 2010 51.88792 0.606184 7.1 55 0.60 8.2 0.72  
CheAbB Chelmer 2010 51.84596 0.423716 1.2 50 0.06 7.7 0.75  
Nene           
NenBro’95 Nene 1995 52.24627 -0.77987 1.2 47 0.69 8.3 0.72 [3]
NenBro’99 Nene 1999 1.2 47 0.80 8.3 0.73 [25]
NenEct Nene 2007 52.24234 -0.80281 4.2 51 0.79 8.6 0.73  
Aire           
AirDar Aire 2011 53.79336 -1.54911 2.7 43 0.73 8.6 0.75  
Arun           
AruHor’95 Arun 1995 51.05516 -0.36197 4.1 54 0.90 7.8 0.73  
AruHor’00 Arun 2000 51.0556 -0.36124 4.1 34 0.29 7.6 0.73 [25]
AruHor’08 Arun 2008 4.1 69 0.73 7.9 0.74 [26]
AruHUS Arun 1995 51.05953 -0.35326 0.2 48 0.01 7.4 0.73 [3]
Thames catchment           
BlaEvH’10 Blackwater 2010 e   4.2 41 0.98 8.5 0.72  
BlaEvH’00 Blackwater 2000 51.327864 -0.769635 8.8 47 0.98 8.3 0.71 [25]
BouChe’11 Bourne 2011 51.38086 -0.47711 4.8 56 0.62 8.3 0.74  
BouChe’02 Bourne 2002 51.40286 -0.54223 5.8 31 0.55 8.8 0.75  
BouChe’06 Bourne 2006 51.40330 -0.54150 5.8 48 0.25 8.3 0.75 [26]
GadCas Gade 2010 51.65893 -0.42559 NM f 56 0.38 8.1 0.74  
KenBul Kennet 2010 51.39707 -1.28485 0.6 51 0.82 8.4 0.74  
KenFou Kennet 2010 51.43564 -0.97664 8.1 32 0.64 8.7 0.75  
KenNor Kennet 2010 51.40165 -1.33725 0.2 52 0.62 8.5 0.73  
LamSha Lambourn 2011 51.40816 -1.30843 0.03 41 0.10 6.8 0.72  
LeeEss Lee 2010 51.77279 -0.18818 6.6 56 0.60 8.3 0.73  
LeeHyd Lee 2010 51.83958 -0.35825 10.3 28 0.36 8.2 0.73  
LeeHyd Lee 1995 51.84751 -0.37111 11.6 44 0.62 7.7 0.70 [3]
LeeHUS Lee 1995 51.84935 -0.37395 NM 37 0.001 7.0 0.73 [3]
LeeSta Lee 2010 51.7894 -0.22496 6.6 31 0.85 8.4 0.71  
Lee’00 Lee 2000 g   NM 41 0.90 8.1 0.70 [25]
LeeWhe Lee 2010 51.81424 -0.28903 6.6 55 0.05 8.5 0.75  
MolMea Mole 2010 51.19028 -0.18581 5.8 42 0.84 8.4 0.73  
RayRod Ray 2003 51.57093 1.81815 10.9 30 0.40 7.7 0.72  
StoBri Stort 2010 51.77989 0.05024 4.1 52 0.92 8.1 0.71  
StoTed Stort 2010 51.83115 0.16892 6.0 30 0.97 8.2 0.69  
ThaCul Thames 2010 51.65046 -1.26739 1.6 44 0.45 8.8 0.74  
ThaHam Thames 2010 51.55989 -0.87347 1.8 44 0.45 8.2 0.74  
ThaWhi Thames 2010 51.48662 1.08974 1.5 60 0.88 8.2 0.74  
ThaSha Thame 2010 51.75281 -1.03319 1.9 50 0.38 7.9 0.74  
WanMoh Wandle 2011 51.40329 0.18821 3.3 48 0.33 8.2 0.75  
Trent           
TreWol Trent 1995 52.781178 -1.971789 3.7 45 0.55 8.0 0.72  
TreNot Trent 2007 g    24 0.50 8.9 0.75  
  1. Full diversity statistics for each locus are given in Additional file 8 and statistical differences in genetic diversity are given in Additional file 3. aHeadings in bold indicate different catchments; bEstradiol equivalents, the predicted average estrogenicity at the sample site, calculated from the predicted concentrations of E1, E2 and EE2;cTPM = two phase model for microsatellite evolution used for this test; dSource of samples; eA composite sample of fish caught at BlaEve (51.354119, -0.8584853) and BlaHaw (51.324119, -0.7665606). Effluent concentrations are an average between the two sites for statistical analysis;
  2. fNM = not modelled. For this site there are no major upstream discharges. For statistical analysis, and average E2Eq value for sites with no major discharges was used. gThe exact locations for these sample sites are unknown. Numbers in bold indicate significance (p ≤ 0.05).