Figure 4From: Pax6 regulates the formation of the habenular nuclei by controlling the temporospatial expression of Shhin the diencephalon in vertebratesThe partition of p2 to form the habenula and the thalamus is controlled by the interaction between Pax6 and Shh . (A–C) Immunofluorescence for GABA, GFP, and neurofilament (NF) on sagittal sections of E14.5 embryos of indicated genotypes. (A’-C’) the blue channel of images A-C to show for NF staining. The dashed line outlines the habenula (Hb); arrows indicate the FR tract; the asterisk shows defasciculating axonal fibers in place of the FR tract. Note that the FR tract becomes enlarged and supernumerary in ShhSG/SG embryos (see inset in C’). Scale bar in (A): 200 μm (A-C’). E, embryonic day; FR, fasciculus retroflexus; GABA, gamma-aminobutyric acid; GFP, green fluorescent protein.Back to article page