Figure 9

Analysis of growth and cell cycle progression following RNAi of Tb 10.NT.87 . (A) Tb10.NT.87 RNAi in procyclics (PC). Growth for uninduced (-tet) and induced (+tet) cells shown in log scale. (B) Cell cycle progression in procyclic cells after RNAi of Tb10.NT.87. Cells were scored every 24 hours as having normal DNA (one kinetoplast and one nucleus (1K1N), two kinetoplasts and one nucleus (2K1N) or two kinetoplasts and two nuclei (2K2N)) or abnormal DNA content (one kinetoplast and no nucleus (1K0N), one kinetoplast and two nuclei (1K2N) and more than two nuclei (>2 N)). (C) Example images of abnormal morphology noted after RNAi of Tb10.NT.87. Hoechst staining and DIC are shown. (D) Examination of kDNA size after Tb10.NT.87 RNAi in procyclic cells. Cells were scored as having normal kDNA size, small kDNA, absent kDNA or asymmetrical kDNA, which is one cell with two daughter kDNA of different sizes, by visually comparing the kDNA size to that in the uninduced control. (E) Examples of cells with normal kDNA, small kDNA (both in G1 and dividing) and absent kDNA. Hoechst and DIC images are displayed. Cell counts were done as described in the legend for Figure 5. DIC, differential interference contrast.