Behavior of ENCCs at different locations. A. An example of a preparation of E12.5 colon. The location of the most caudal cell is indicated with a yellow dotted line. B. The speed of ENCCs. ENCCs between 750 and 1,500 μm from the wavefront migrated significantly slower than those within 450 μm of the most caudal cell. C. The angle of migration between the beginning of end of imaging for each cell, relative to the long axis of the gut, which was defined as 0°. Only angles between 0 and 180° were used. D. Tortuosity; there were no significant differences between the tortuosity of ENCCs at different locations. E. Rate of caudal advance was defined as the longitudinal distance an ENCC advanced caudally during the imaging period regardless of the route (see Figure 2F). ENCC < 150 μm and > 750 μm from the most caudal cell showed the lowest rates of caudal advance. All data are shown as mean ± s.e.m. and were analyzed using ANOVAs followed by Tukey’s post-hoc tests. Asterisks indicate P < 0.05. ENCCs, enteric neural crest-derived cells.