Spatial order of cells within 1 mm of the migratory wavefront in the E12.5 colon. A. The small group of cells (arrow) that were in contact with each other when photoconverted. B. Tracks of six of the cells in the subsequent five hours showing that they migrated in a variety of directions. C-E. A different E12.5 colon explant to show intermixing of ENCCs. C’. Red and green channels showing photoconversion of four groups of cells, which were 80, 200, 600 and 1,000 μm from the most caudal cell at the commencement of imaging. C. Red channel only showing the extents of initial locations of each group, which are marked by the pale green, yellow, blue and pink bars. D, E The spatial extents of cells two hours (D) and four hours (E) later are shown by the darker green, yellow, blue and red bars. The darker lines indicate the furthest extent of dispersal of cells from each group, not the number of cells; more cells dispersed caudally than rostrally from each group of photoconverted cells. ENCCs, enteric neural crest-derived cells.