Wound healing, a necessary precursor to regeneration. (A) Wound healing and regeneration are separable developmental processes that may involve different gene cascades. (B) Head regeneration is a rapid process in Nematostella where structural integrity is renewed approximately 72 hours after injury. (C) Schematic representation of the experimental setup of regeneration and wound healing experiments using Nematostella juveniles. In regeneration experiments, animals are cut just below the pharynx region (red line). In wound healing experiments, animals are punctured at the central position between the mesenteries and the aboral pole. Puncture wounds span both sides of the animal creating two similar wounds.