Similar activation of wound healing transcripts during head regeneration. All five genes identified by puncture assays were also up-regulated during wound healing, prior to regeneration. (A) Time series of expression levels of uromodulin transcripts during oral and aboral wound healing events. One hour after injury, uromodulin localizes to the aboral pole of tissue that is regenerating oral structures, and around the base of the tentacles in tissue regenerating aboral structures. U0126 blocks the normal activation of uromodulin during regeneration. Uromodulin appears to be highly expressed in the aboral ectoderm during both oral and aboral regeneration. (B) All four genes exhibit the same expression domains as previously identified during puncture analysis. SoxE1 and the thiamine enzyme are both expressed along the ectoderm at the injury site in both oral and aboral regenerates. Similar to puncture assays, the matrix metalloproteinase (MMP) inhibitor is highly expressed in the endoderm at the site of injury in a circle pattern. The maltase enzyme also localizes to the endoderm in both oral and aboral regenerates. Incubation of tissue in U0126 appears to reduce expression in all samples. All time points contain a lateral view of the aboral regenerate, an oral-aboral view of the oral regenerate, and a lateral view of an oral regenerate. (Red star indicates the position of the oral side of the animal, red line indicates circular expression).