VAPB/ALS8 interacts with FFAT-like proteins including the p97 cofactor FAF1 and the ASNA1 ATPase

Table 4 Ubiquitinated targets of VAPB and FAF1 identified by mass spectrometry upon enrichment of ubiquitinated peptides

Protein name	UniProt ID	GlyGly peptides^a(times identified)^b
RPN2	P04844	LSK¹⁵⁴EETVLATVQALQTASHLSQQADLR (4×)
		K²⁴⁴NFESLSEAFSVASAAAVLSHNR (2×)
		LHNQK⁴⁴²TGQEVVFVAEPDNK (3×)
DDOST	P39656	APTIVGK¹⁸⁹SSLNPILFR (2×)

^aA mixture of light-labeled Flag-VAPB and heavy-labeled Flag-FAF1 immunoprecipitates was analyzed by mass spectrometry after ubiquitinated peptide enrichment using antibodies specific to lysine-ϵ-GlyGly. The lysine residues carrying the GlyGly modification are indicated in bold and the superscript is the position in the protein sequence.
^bThe numbers in parenthesis indicate how many times each peptide was identified by mass spectrometry.

ISSN: 1741-7007