VAPB/ALS8 interacts with FFAT-like proteins including the p97 cofactor FAF1 and the ASNA1 ATPase

Table 7 FFAT-like proteins identified in VAPA and VAPB immunoprecipitates by mass spectrometry

Protein name	UniProt ID^a	MW (Da)^a	FFAT motif^a	Share of spectrum IDs^b
				Flag-VAPA (U2OS)	Flag-VAPB (U2OS)	Endogenous VAPB
				Flag-VAPA (U2OS)	Flag-VAPB (U2OS)	HeLa cells	Mouse brain
FAF1	Q9UNN5	73954	DFEDATE	0.85%	0.68%	0.06%	-
ASNA1	O43681	38793	EFEDAPD	0.69%	0.79%	0.20%	-
RAB3GAP1	Q15042	110524	EFFECLS	2.65%	1.79%	0.20%	0.43%
RAB3GAP2	Q9H2M9	155985	NA	1.73%	1.61%	0.10%	0.37%
WDR44	Q5JSH3	101366	EFYDAPE	1.97%	1.66%	-	0.05%
STX1B ^c	P61266	33245	EFFEQVE	-	-	-	0.20%
STX1A ^c	Q16623	33023	EFFEQVE	-	-	-	0.03%

^aThe UniProt ID, MW and FFAT motif are indicated for the human proteins.
^bThe share of spectrum IDs is indicated as a measure of protein abundance in the immunoprecipitates.
^cSTX1A and B are shown in italics because their putative FFAT-like motifs could not be confirmed experimentally.
NA, not applicable.

ISSN: 1741-7007