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Figure 4 | BMC Biology

Figure 4

From: Six1 is a key regulator of the developmental and evolutionary architecture of sensory neurons in craniates

Figure 4

Appearance of intramedullary sensory neuron-like cells in Six1/4EGFP/EGFPmice. (A-H) Magenta represents rhodamine-dextran injected outside the spinal cord (sc) (A,B), TLX3 (C,D), KCNA1 (E,F), RUNX1 (G,H) and (I,J) ISL1/2, which are detected in intramedullary enhanced GFP (EGFP)-positive cells (green) in Six1/4EGFP/EGFP spinal cord. Arrowheads: double-positive cells. (K) Intramedullary EGFP-positive cells (green) are labeled with neural crest cell (NCC) lineage-specific fluorescence of monomeric RFP (magenta). (L) EGFP (green)- and ISL1/2 (magenta)-double-positive intramedullary cells disappear with NCC-specific restoration of SIX1. (M) Number of EGFP- and ISL1/2-double-positive cells in the spinal cord at the level of somite 21 to 26. (N) Schematic representation of dorsal root ganglion (DRG) neurons (blue) and intramedullary neuron-like cells (pink) in Six1/4+/EGFP and Six1/4EGFP/EGFP. A-F and I-L are on embryonic day (E)11.5; G and H are on E 12 . A-H, K and L are transverse sections (top: dorsal side); I and J are coronal sections (top: lateral side). Data are mean ± standard error of the mean. *p <0.001. Dashed lines demarcate the position of ectoderm and sc. Scale bars: 50 μm.

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