RNA-seq: impact of RNA degradation on transcript quantification

Table 2 Number of identified DE genes

	GLM: reads approximate time point
Time point	GLM	GLM + RIN	Regress RIN, GLM
0 h versus 12 h	608	5	26
0 h versus 24 h	3,704	5	203
0 h versus 48 h	8,756	47	5
0 h versus 84 h	9,998	42	0
	GLM: reads approximate individual b
Individuals	GLM	GLM + RIN	Regress RIN, GLM
Ind 1 versus Ind 3	69	553	268
Ind 1 versus Ind 4	48	401	190
Ind 3 versus Ind 4	100	573	299

b, Treating time as technical replicates; DE, Differentially expressed; GLM, generalized linear model; H, hours; RIN, RNA integrity number.

ISSN: 1741-7007