Figure 4

In vitro and in vivo activity of Wnt3a NTD and CTD constructs. (A) Schematic representation of applied mouse Wnt3a NTD and CTD constructs. (B) Wnt reporter assay in HEK293T showing the effect of the Wnt3a NTD and CTD on signaling activity. Experiments were carried out in triplicate. Error bars depict standard deviation. Statistical significance in relative luciferase activity levels compared to wild-type Wnt3a levels as indicated: *P < 0.05, **P < 0.01, ***P < 0.001 and n.s. as not significant according to Student’s t-test. (C-G) Xenopus embryos at 4-cell stage were injected into the ventral-marginal-zone with 3pg mRNA encoding wild type Wnt3a (D), NTD (E), CTD (F) or NTD + CTD (G).