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Table 1 Activity of Wnt3a mutants

From: Molecular dissection of Wnt3a-Frizzled8 interaction reveals essential and modulatory determinants of Wnt signaling activity

Wnt3a mutants

Wnt reporter assay

Fz8 binding

Zebrafish embryo

wt Wnt3a

+ +

+ +

Abnormal

Site 1

   

K204A

-

nd

nd

L208A

-

nd

nd

S209A

- - -

- -

94% normal

G210R

- - -

- -

75% normal

W218A

-

nd

nd

Site 2

   

F331A

- -

+ +

Same as wt

W333A

- -

+

Same as wt

C335A

- - -

+

Same as wt

Y336A

+ +

nd

nd

V337A

+ +

nd

Same as wt

V337D

- - -

nd

50% normal

V337I

-

nd

70% normal

V337R

- - -

+ +

92% normal

V337Y

- - -

nd

92% normal

V337W

- - -

nd

94% normal

Pseudo site 3

   

I45R

+ + +

nd

nd

Y59A

+ + +

nd

nd

V60R

- -

+

Same as wt

M63A

+ + +

nd

nd

P64A

+ + +

nd

nd

A67R

+ + +

nd

nd

E68A

- -

+

nd

S92A

+ + +

nd

nd

N93A

+ + +

nd

nd

A96R

+ + +

+ +

Same as wt

F169A

- -

- -

Same as wt

R173A

+ + +

nd

nd

N175A

-

nd

nd

  1. Effect of Wnt3a mutants tested in TCF/Wnt-reporter, Fz8-CRD binding and zebrafish embryo assays. For the Wnt reporter assay, + +, + + +, -, - - and - - - represent, high (wild type), higher than wild type, lower than wild type, very low and no signaling activity. For Fz8-CRD binding, + +, + and - - represent full, low and no binding. For the zebrafish embryo assay, wt Wnt3a showed an abnormally dorsalized phenotype, including defects in the formation of the anterior-most neural system.
  2. nd, not determined; wt wild type.