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Table 1 Activity of Wnt3a mutants

From: Molecular dissection of Wnt3a-Frizzled8 interaction reveals essential and modulatory determinants of Wnt signaling activity

Wnt3a mutants Wnt reporter assay Fz8 binding Zebrafish embryo
wt Wnt3a + + + + Abnormal
Site 1    
K204A - nd nd
L208A - nd nd
S209A - - - - - 94% normal
G210R - - - - - 75% normal
W218A - nd nd
Site 2    
F331A - - + + Same as wt
W333A - - + Same as wt
C335A - - - + Same as wt
Y336A + + nd nd
V337A + + nd Same as wt
V337D - - - nd 50% normal
V337I - nd 70% normal
V337R - - - + + 92% normal
V337Y - - - nd 92% normal
V337W - - - nd 94% normal
Pseudo site 3    
I45R + + + nd nd
Y59A + + + nd nd
V60R - - + Same as wt
M63A + + + nd nd
P64A + + + nd nd
A67R + + + nd nd
E68A - - + nd
S92A + + + nd nd
N93A + + + nd nd
A96R + + + + + Same as wt
F169A - - - - Same as wt
R173A + + + nd nd
N175A - nd nd
  1. Effect of Wnt3a mutants tested in TCF/Wnt-reporter, Fz8-CRD binding and zebrafish embryo assays. For the Wnt reporter assay, + +, + + +, -, - - and - - - represent, high (wild type), higher than wild type, lower than wild type, very low and no signaling activity. For Fz8-CRD binding, + +, + and - - represent full, low and no binding. For the zebrafish embryo assay, wt Wnt3a showed an abnormally dorsalized phenotype, including defects in the formation of the anterior-most neural system.
  2. nd, not determined; wt wild type.