Wnt3a mutants | Wnt reporter assay | Fz8 binding | Zebrafish embryo |
---|---|---|---|
wt Wnt3a | + + | + + | Abnormal |
Site 1 | |||
K204A | - | nd | nd |
L208A | - | nd | nd |
S209A | - - - | - - | 94% normal |
G210R | - - - | - - | 75% normal |
W218A | - | nd | nd |
Site 2 | |||
F331A | - - | + + | Same as wt |
W333A | - - | + | Same as wt |
C335A | - - - | + | Same as wt |
Y336A | + + | nd | nd |
V337A | + + | nd | Same as wt |
V337D | - - - | nd | 50% normal |
V337I | - | nd | 70% normal |
V337R | - - - | + + | 92% normal |
V337Y | - - - | nd | 92% normal |
V337W | - - - | nd | 94% normal |
Pseudo site 3 | |||
I45R | + + + | nd | nd |
Y59A | + + + | nd | nd |
V60R | - - | + | Same as wt |
M63A | + + + | nd | nd |
P64A | + + + | nd | nd |
A67R | + + + | nd | nd |
E68A | - - | + | nd |
S92A | + + + | nd | nd |
N93A | + + + | nd | nd |
A96R | + + + | + + | Same as wt |
F169A | - - | - - | Same as wt |
R173A | + + + | nd | nd |
N175A | - | nd | nd |