Figure 2

Microtubule growth speed is increased in the growth cone of conditioned DRG neurons. (A) Kymographs of single processes of naïve and conditioned (CL) neurons transfected with EB3-GFP. Scale bar: 1 μm. (B) Quantification of microtubule growth speed in naïve and in conditioned (CL) neurons transfected with EB3-GFP; (n = 140 to 170 microtubules). (C) Quantification of EB3-fluorescence intensity relative to the distance to the growth cone tip in naïve and in conditioned (CL) neurons transfected with EB3-GFP; (n = 10 growth cones). For distances closer to the leading edge (between 0.48 and 4.48 μm) the differences presented are statistically significant with P <0.05. (D) Representative images of growth cones of naïve and in conditioned (CL) neurons transfected with EB3-GFP. The green line surrounds the leading edge of the cell. Scale bar: 5 μm. All error bars are SEM. ***P <0.001. Two-tailed Student’s t test. CL, conditioned lesion; DRG, dorsal root ganglia; SEM, standard error of the mean.