Figure 8

The GSK3β-CRMP-2 pathway participates in the conditioning lesion effect. (A) Quantification of microtubule growth speed in naïve and conditioned (CL) DRG neurons transfected with EB3-GFP, or conditioned neurons co-transfected with EB3-GFP and T/D-CRMP-2 (CL + T/D-CRMP-2); (n = 140 to 170 microtubules). (B) Quantification of the average size of the longest neurite in naïve and conditioned (CL) DRG, or conditioned neurons overexpressing T/D-CRMP-2 (CL + T/D-CRMP-2); (n = 40 to 91). (C) Representative images of C. Scale bar: 100 μm. (D) Quantification of the percentage of cells with neurites in naïve and conditioned (CL) DRG, or conditioned neurons overexpressing T/D-CRMP-2 (CL + T/D-CRMP-2). (E) Quantification of the size of the longest neurite of DRG neurons plated on top of laminin (control) or laminin + myelin (myelin), either transfected with pEGFP-C1 or co-transfected with pEGFP-C1 and T/A-CRMP-2 (myelin + T/A-CRMP-2); (n = 64 to 83). (F) Representative images of F. Scale bar: 30 μm. All error bars are SEM. *P <0.05. **P <0.01. ***P <0.001. One way ANOVA. ANOVA, analysis of variance; CRMP, collapsin response mediator protein; DRG, dorsal root ganglia; GFP, green fluorescent protein; GSK3β, glycogen synthase kinase 3β; SEM, standard error of the mean.