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Figure 5 | BMC Biology

Figure 5

From: Larval body patterning and apical organs are conserved in animal evolution

Figure 5

Azakenpaullone treatment of embryos alters gene expression in the episphere in a dose-dependent manner. All images are apical views with dorsal at the top of the image and ventral at the bottom. (A) Six3 is severely reduced in embryos treated with azakenpaullone. (B) Foxq2 expression is lost in all but the most apical cells of the episphere in azakenpaullone treatments. (C) Fewer otp cells are stained at increasing concentrations of azakenpaullone and expression is entirely lost at 10 μM. (D) Hox1, a molecular marker of the ampullary cells (arrows), is not affected by azakenpaullone treatment. Ampullary cells are specified very early in development, earlier than the initiation of chemical treatments at 12 hours post-fertilization, prior to the differentiation of the remainder of the episphere. (E) Pax6 expression, which marks the ventral regions of the developing apical plate, is expanded in the episphere upon treatment with azakenpaullone. Counts of the embryos displaying wild-type, reduced or expanded expression domains for two replicates at 0, 1 and 5 μM concentrations are displayed in Figure S4.

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