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Table 1 Effect of combining the exonuclease and active site mutations in Pol ε gene on mutation rates in haploids in 8C-YUNI101 genetic background

From: Evidence for interplay among yeast replicative DNA polymerases alpha, delta and epsilon from studies of exonuclease and polymerase active site mutations

POL2 allele Mutation rates for different markers*
  Forward mutation Reversion
   Base substitutions Frameshifts (+1)
  Canr (× 10-7) Ura+ × (10-8) His+ × (10-8)
Mismatch repair proficient strains
wild-type 3.3 1.3 1.1
  2.5–4.9 1.1–1.7 0.9–3.5
pol2-Y831A 5.1 1.2 1.6
  3.5–12.1 0.8–2.8 0.5–2.7
pol2-4 27 3.41 452
  15–42 2.4–5.7 35–78
pol2-4,Y831A 9 1.5 3.93
  7–15 1.1–1.9 2.3–5.4
Mismatch repair deficient strains (pms1)
wild-type 90 2.8 87
  72–150 2.4–4.1 54–150
pol2-Y831A 340 124 4705
  230–420 11–19 270–790
pol2-4 760 1606 16007
  120–1060 90–420 950–2800
pol2-4,Y831A 270 158 4109
  190–440 8–55 260–440
  1. *Here and thereafter, median for 9–27 independent cultures with 95% confidence limits below. 114/16 of revertants sequenced were due to C->A transversions 220/20 revertants sequenced contained a +1A frameshift event 316/16 revertants sequenced were due to +1A frameshift 412/14 revertants were due to C->A change 515/15 revertants sequenced were due to +1A frameshift 612/12 revertants sequenced were due to C->A mutation 716/16 revertants sequenced were due to +1A frameshift 812/15 revertants sequenced were due to C->A mutation 914/14 revertants were due to +1A frameshift
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