|
POL2 allele
|
Mutation rates for different markers*
|
|---|
| |
Forward mutation
|
Reversion
|
|---|
| | |
Base substitutions
|
Frameshifts (+1)
|
|---|
| |
Canr (× 10-7)
|
Ura+ × (10-8)
|
His+ × (10-8)
|
|---|
|
Mismatch repair proficient strains
|
|
wild-type
|
3.3
|
1.3
|
1.1
|
| |
2.5–4.9
|
1.1–1.7
|
0.9–3.5
|
|
pol2-Y831A
|
5.1
|
1.2
|
1.6
|
| |
3.5–12.1
|
0.8–2.8
|
0.5–2.7
|
|
pol2-4
|
27
|
3.41
|
452
|
| |
15–42
|
2.4–5.7
|
35–78
|
|
pol2-4,Y831A
|
9
|
1.5
|
3.93
|
| |
7–15
|
1.1–1.9
|
2.3–5.4
|
|
Mismatch repair deficient strains (pms1)
|
|
wild-type
|
90
|
2.8
|
87
|
| |
72–150
|
2.4–4.1
|
54–150
|
|
pol2-Y831A
|
340
|
124
|
4705
|
| |
230–420
|
11–19
|
270–790
|
|
pol2-4
|
760
|
1606
|
16007
|
| |
120–1060
|
90–420
|
950–2800
|
|
pol2-4,Y831A
|
270
|
158
|
4109
|
| |
190–440
|
8–55
|
260–440
|
- *Here and thereafter, median for 9–27 independent cultures with 95% confidence limits below. 114/16 of revertants sequenced were due to C->A transversions 220/20 revertants sequenced contained a +1A frameshift event 316/16 revertants sequenced were due to +1A frameshift 412/14 revertants were due to C->A change 515/15 revertants sequenced were due to +1A frameshift 612/12 revertants sequenced were due to C->A mutation 716/16 revertants sequenced were due to +1A frameshift 812/15 revertants sequenced were due to C->A mutation 914/14 revertants were due to +1A frameshift
