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Table 2 Effect of combining the exonuclease and active site mutations in DNA polymerase ε on mutation rates in diploids. Mismatch repair proficient strains

From: Evidence for interplay among yeast replicative DNA polymerases alpha, delta and epsilon from studies of exonuclease and polymerase active site mutations

Relevant mutation His+ reversion rate (× 10-8) Fold increase
+/+ 0.5 1
  0.3–1.2  
+/ pol2-Y831A 1.1 2.2
  0.7–1.7  
pol2-Y831A/ pol2-Y831A 2.2 4.4
  1.8–5.7  
+/pol2-4 3.8 7.6
  2.6–4.7  
pol2-4/pol2-4 26 52
  16–45  
pol2-4/ pol2-Y831A 28 56
  16–53  
pol2-4/ pol2-4,Y831A 21 42
  18–34  
pol2-Y831A/ pol2-4,Y831A 1.8 3.6
  1.2–3.1  
pol2-4,Y831A/pol2-4,Y831A 1.8 3.6
  1.1–2.3  
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