Table 3 Effect of combining the exonuclease and active site mutations in DNA polymerase ε on mutation rates in diploids. Mismatch repair deficient strains (pms1/pms1)
Relevant mutation | His+ reversion rate (× 10-8) | Fold increase |
|---|---|---|
+/+ | 110 | 1 |
|  | 90–140 |  |
+/pol2-Y831A | 400 | 4 |
|  | 270–600 |  |
pol2-Y831A/pol2-Y831A | 820 | 7 |
|  | 750–950 |  |
+/pol2-4 | 1,500 | 14 |
|  | 930–3600 |  |
pol2-4/pol2-4 | 9,400 | 85 |
|  | 5,900–14,000 |  |
pol2-4/ pol2-Y831A | 8,400 | 76 |
|  | 3,500–17,000 |  |
+/pol2-4,Y831A | 210 | 2 |
|  | 180–270 |  |
pol2-4/pol2-4,Y831A | 11,000 | 100 |
|  | 8,100–22,000 |  |
pol2-Y831A/pol2-4,Y831A | 1,200 | 11 |
|  | 1,030–1,460 |  |
pol2-4,Y831A/pol2-4,Y831A | 1,020 | 9 |
|  | 840–2,300 |  |