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Table 5 Effect of combining the mutation in exonuclease domains of DNA polymerases δ and ε genes with active site mutation in DNA polymerases α and δ genes on mutation rates in mismatch repair proficient haploid derivatives of 8C-YUNI101

From: Evidence for interplay among yeast replicative DNA polymerases alpha, delta and epsilon from studies of exonuclease and polymerase active site mutations

Relevant mutation Mutation rates
  Forward Canr mutation Ura+ reversion (base substitutions) His+ reversion (+ 1 frameshifts)
  Rate (× 10-7) Fold increase Rate (× 10-8) Fold increase Rate (× 10-8) Fold increase
no 4.4 1 1.3 1 1.9 1
  2.9–5.8   1.2–1.7   1.4–3.6  
pol1-Y869A 12 2.7 1.1 0.8 6.4 3.3
  6.2–28   0.8–2.2   4.1–16  
pol2-4 17 3.8 5.1 3.9 51 27
  12–24   3.7–5.5   45–71  
pol2-4 pol1-Y869A 46 10 8.8 6.7 120 63
  41–58   4.6–11   77–160  
pol3-01 190 43 19 15 110 59
  136–260   6–35   80–160  
pol3-01 pol1-Y869A 220 50 34 26 114 60
  140–240   27–48   87–210  
pol3-Y708A 28 6.3 6.1 4.7 6.0 3.1
  14–32   5.0–7.7   4.8–11  
pol2-4 pol3-Y708A 92 21 15 12 73 38
  73–120   14–18   61–104  
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