Table 5 Effect of combining the mutation in exonuclease domains of DNA polymerases δ and ε genes with active site mutation in DNA polymerases α and δ genes on mutation rates in mismatch repair proficient haploid derivatives of 8C-YUNI101
Relevant mutation | Mutation rates | |||||
|---|---|---|---|---|---|---|
| Â | Forward Canr mutation | Ura+ reversion (base substitutions) | His+ reversion (+ 1 frameshifts) | |||
|  | Rate (× 10-7) | Fold increase | Rate (× 10-8) | Fold increase | Rate (× 10-8) | Fold increase |
no | 4.4 | 1 | 1.3 | 1 | 1.9 | 1 |
|  | 2.9–5.8 |  | 1.2–1.7 |  | 1.4–3.6 |  |
pol1-Y869A | 12 | 2.7 | 1.1 | 0.8 | 6.4 | 3.3 |
|  | 6.2–28 |  | 0.8–2.2 |  | 4.1–16 |  |
pol2-4 | 17 | 3.8 | 5.1 | 3.9 | 51 | 27 |
|  | 12–24 |  | 3.7–5.5 |  | 45–71 |  |
pol2-4 pol1-Y869A | 46 | 10 | 8.8 | 6.7 | 120 | 63 |
|  | 41–58 |  | 4.6–11 |  | 77–160 |  |
pol3-01 | 190 | 43 | 19 | 15 | 110 | 59 |
|  | 136–260 |  | 6–35 |  | 80–160 |  |
pol3-01 pol1-Y869A | 220 | 50 | 34 | 26 | 114 | 60 |
|  | 140–240 |  | 27–48 |  | 87–210 |  |
pol3-Y708A | 28 | 6.3 | 6.1 | 4.7 | 6.0 | 3.1 |
|  | 14–32 |  | 5.0–7.7 |  | 4.8–11 |  |
pol2-4 pol3-Y708A | 92 | 21 | 15 | 12 | 73 | 38 |
|  | 73–120 |  | 14–18 |  | 61–104 |  |