In general, the ellipsoid's main axes along x'y'z' do not coincide with the microscope-slide plane and optical axes xyz. Our programme finds an ellipsoid that contains all the telomeres and the size of its main axes a,b,c. In most of the cases the x'y' axes of the ellipsoid are similar, i.e. a≈b. Therefore, the ratio a/c is a good measure of the flatness level of the ellipsoid and of the telomere organization inside the nucleus.