Figure 4

Quantitative reverse transcriptase PCR analysis of transcript abundance in the gluteus medius and supraspinatus muscles. Least square means and standard errors for transcript abundance by genotype are shown for the gluteus medius (GM), and supraspinatus (SP). The genotypes are given with the maternal allele first followed by the paternal allele and the CLPG allele has been abbreviated to C. Quantification of DLK1 and MEG8 transcripts (A, C) was based on random primed cDNA synthesis using 213 ng of total RNA. Quantification of PEG11 and PEG11AS (B, D) transcripts was based on gene-specific priming of cDNA synthesis using 400 ng of total RNA. Different superscripts indicate significant differences (p < 0.05) between genotypic means for a given mRNA transcript and muscle. Numerical values for the log starting abundance are given for each mRNA transcript in a table below the genotypes.