Table 2 Primer sequences for ribonuclease protection assay probes and quantitative PCR

From: Expression of PEG11 and PEG11AS transcripts in normal and callipyge sheep

			Size^a	T_m	Location^b
Primer		Sequence	bp	°C
PEG11/PEG11AS
C	F	AGGAACACCGCTGTGGAGGTAGAA	135	59	4337
	R	ACAGCAGAGGCAGCCAAGCA
E	F	GGTGACGCCCGTCTGCAAGT	180	61	2427
	R	GTGGAACGGTTCGCCGACAT
P^c	F	ACAGCTCAACAGTGGAGGTCATG	199	55	996
	R	ATCAGCTGGCAGAGCACGATGAAC
F	F	TTCCCCCATGGCTGTGAGAAAT	175	58	-390
	R	CCTCTGTGACCTTCTGGTGACCAA
G	F	AGGCTGAATTGACAGAGATGT	124	49	-1193
	R	GTTAAATGGCTCAAGAACGA
DLK1	F	CCCGTCCTCTTGCTCCTGCT	116	58
	R	GGCTGGCACCTGCACACACT
MEG8	F	CCCAGGGAGTGTGAGGCTCTTCT	100	56
	R	GGACCCACGGCTGACCTGTT
G3PD	F	TGAGTGTCGCTGTTGAAGT	150	58
	R	CCTGCCAAGTATGATGAGAT

^aSize of the PCR product and protected fragment for RPA ^bLocation for the PCR product is relative to the first base of the PEG11 open reading frame. ^cCharlier et al. [3]

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ISSN: 1741-7007

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