Southern blot analysis of Stu I-digested DNAs of wild-type Nectria haematococca and five transformants. Total genomic DNAs of wild-type (wt) Nectria haematococca and five s transformants (Tr1-Tr5) that were obtained after co-transformation of wt with psec3-s1 plasmid and pBC-Hygro vector, and probed with psec3-s1. All the transformants share a large band (>15 kb), absent from wild type. Tr3 and Tr5 contain both the wild type and the s
1 allele. The faint bands noted * correspond to repeated sequences homologous to those present at the Ses locus.