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Table 1 Effects of genotype and drug on [3H]DA uptake and DAT density

From: Low dose pramipexole is neuroprotective in the MPTP mouse model of Parkinson's disease, and downregulates the dopamine transporter via the D3 receptor

    [3H]DA uptake DAT density  
Group N Vmax SD Km SD DAT SD
WT w Veh 4 516.1 ± 56.5 56.7 ± 18.6 10.6 ± 2.5
D3 KO w Veh 4 212.4 ± 18.2 10.8 ± 5.7 10.8 ± 1.5
WT w PPX 6 183.4 ± 34.3 28.4 ± 18.5 9.9 ± 3.0
D3 KO w PPX 6 223.6 ± 36.4 10.4 ± 10.9 9.4 ± 2.6
  1. Wild-type and D3 KO mice were administered vehicle (0.9% saline) or pramipexole (PPX, 0.1 mg/kg I.P.) once a day for 5 days. Twenty-four hours after the last injection the brains were removed, the CPu dissected and fresh frozen ([3H]DA uptake) or brains frozen on dry-ice ([125I] RTI-55 autoradiography).
  2. [3H]DA uptake Vmax reported as pmol/mg P/5 min and Km reported as nM. [125I] RTI-55 autoradiography for DAT density reported as fmol/μg P.
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