Wild-type and D3 KO mice were administered vehicle (0.9% saline) or pramipexole (PPX, 0.1 mg/kg I.P.) once a day for 5 days. Twenty-four hours after the last injection the brains were removed, the CPu dissected and fresh frozen ([3H]DA uptake) or brains frozen on dry-ice ([125I] RTI-55 autoradiography).
[3H]DA uptake Vmax reported as pmol/mg P/5 min and Km reported as nM. [125I] RTI-55 autoradiography for DAT density reported as fmol/μg P.