| | | |
[3H]DA uptake
|
DAT density
| |
|---|
|
Group
|
N
|
Vmax
|
SD
|
Km
|
SD
|
DAT
|
SD
|
|---|
|
WT w Veh
|
4
|
516.1
|
± 56.5
|
56.7
|
± 18.6
|
10.6
|
± 2.5
|
|
D3 KO w Veh
|
4
|
212.4
|
± 18.2
|
10.8
|
± 5.7
|
10.8
|
± 1.5
|
|
WT w PPX
|
6
|
183.4
|
± 34.3
|
28.4
|
± 18.5
|
9.9
|
± 3.0
|
|
D3 KO w PPX
|
6
|
223.6
|
± 36.4
|
10.4
|
± 10.9
|
9.4
|
± 2.6
|
- Wild-type and D3 KO mice were administered vehicle (0.9% saline) or pramipexole (PPX, 0.1 mg/kg I.P.) once a day for 5 days. Twenty-four hours after the last injection the brains were removed, the CPu dissected and fresh frozen ([3H]DA uptake) or brains frozen on dry-ice ([125I] RTI-55 autoradiography).
- [3H]DA uptake Vmax reported as pmol/mg P/5 min and Km reported as nM. [125I] RTI-55 autoradiography for DAT density reported as fmol/μg P.
