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Figure 3 | BMC Biology

Figure 3

From: Acetylation of insulin receptor substrate-1 is permissive for tyrosine phosphorylation

Figure 3

IRS-1 is a lysine acetylated protein. MCF-7 cells were treated with IGF-1 (10 ng/ml) for the indicated times, PMA (10 ng/ml) for 4 or 6 hours as indicated or TSA (150 ng/ml) for 4 hours prior to lysis and immunoprecipitation of IRS-1 Western blots were probed for the presence of acetyl lysine. Densitometric analysis of acetylated IRS-1 was performed and is displayed in the histogram. This experiment has been repeated one other time although the effects of TSA on acetylation of IRS-1 have been observed in many other experiments.

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