Figure 5
From: Acetylation of insulin receptor substrate-1 is permissive for tyrosine phosphorylation
Inhibition of HAT activity inhibits insulin signalling. MCF-7 cells were treated with PMA, TSA and DesCoA (10 nM) in the indicated combinations for four hours prior to stimulation with insulin for 10 minutes. IRS-1 was immunoprecipitated and western blotted for the presence of IRS-1 itself, HDAC2 and phosphotyrosine. The graph shows average data for phosphotyrosine from three independent experiments (± SEM) normalised to the response to insulin alone.