Figure 6 From: Acetylation of insulin receptor substrate-1 is permissive for tyrosine phosphorylation Distal insulin signalling is enhanced by increased IRS-1 lysine acetylation. MCF-7 cells were treated with PMA, TSA and insulin as for Figure 4 and lysates were western blotted for the presence of PKB phosphorylated on serine 474. The graph shows average data from three independent experiments (± SEM) normalised to the insulin response alone.Back to article page