Table 4 Degenerate primers used for the cloning of cDNAs
From: Basal jawed vertebrate phylogeny inferred from multiple nuclear DNA-coded genes
| Target | Primer | Amino acid sequence | |
|---|---|---|---|
| ALDa-c | sense | 5'-CAGGATCCAARGGIATHYTIGCNGC-3' | GKGILAA |
| sense | 5'-GGCCGTCGTCGGNATHAARGTNGA-3' | VGIKVD | |
| antisense | 5'-CAGAATTCGTIACCATRTTIGGYTT-3' | KPNMVT | |
| antisense | 5'-GTGIACGCAICKCCAYTTNGCRAA-3' | FAKWRCV | |
| ALDc | antisense | 5'-CTCYTTYTTNCCNCCCCANGCYTT-3' | KAWGGKKE |
| ALDa,c | antisense | 5'-GGCTAGNGGNACNACNCCYTTRTC-3' | DKGVVPLA |
| G6PD | sense | 5'-CATAATGGGIGCIWSIGGNGAYYT-3' | IMGASGDL |
| sense | 5'-GTCAGCTACTGGNGAYYTNGCNAA-3' | ATGDLAK | |
| sense | 5'-GTCATCTTCGGIGCNWSNGGNGAY-3' | GASGD | |
| sense | 5'-GACCACTAYYTNGGNAARGARATG-3' | DHYLGKEM | |
| antisense | 5'-AGATGCAGGYTTYTCCATNGCNAC-3' | VAMEKPAS | |
| antisense | 5'-GTCGSWNCKNACRAARTGCATYTG-3' | QMHFVRSD | |
| GAG | sense | 5'-CAGTGGCGGIMGIGARCAYRC-3' | SGGREH(A/T) |
| sense | 5'-GATGCCNCCIGCNCARGAYC-3' | MPPAQDH | |
| sense | 5'-GAGTTYAAYTGYMGNTTYGG-3' | EFNCRFG | |
| antisense | 5'-CCGAANCKRCARTTRAAYTC-3' | EFNCRFG | |
| antisense | 5'-GTTRAAGGTNCKIGYCATYTC-3' | EM(A/T)RTFN | |
| antisense | 5'-GAAGCCTGCIARRTCRTAYTC-3' | EYDLAGF | |
| antisense | 5'-GTCRTTIACRCACATNGCNAC-3' | VAMCVND | |
| PGK | sense | 5'-CATCCGIGTNGAYTTYAAYGTNCC-3' | RVDFNVP |
| antisense | 5'-GAAGACACCNGGNGGNCCRTTCCA-3' | WNGPPGVF | |
| antisense | 5'-CTTGTCISWIACYTTNGCNCCNCC-3' | GGAKVSDK | |
| antisense | 5'-GTTCTCDATNARYTGDATYTTRTC-3' | DKIQLI | |
| TPI | sense | 5'-CCGGTACCAAYTGGAARATGAAYGG-3' | NWKMNG |
| antisense | 5'-ATGGATCCCCIACIARRAAICCRTC-3' | DGFLV | |
| antisense | 5'-GCCTATGGCCCANACNGGYTCRTA-3' | YEPVWAIG |