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Table 4 Degenerate primers used for the cloning of cDNAs

From: Basal jawed vertebrate phylogeny inferred from multiple nuclear DNA-coded genes

Target   Primer Amino acid sequence
ALDa-c sense 5'-CAGGATCCAARGGIATHYTIGCNGC-3' GKGILAA
  sense 5'-GGCCGTCGTCGGNATHAARGTNGA-3' VGIKVD
  antisense 5'-CAGAATTCGTIACCATRTTIGGYTT-3' KPNMVT
  antisense 5'-GTGIACGCAICKCCAYTTNGCRAA-3' FAKWRCV
ALDc antisense 5'-CTCYTTYTTNCCNCCCCANGCYTT-3' KAWGGKKE
ALDa,c antisense 5'-GGCTAGNGGNACNACNCCYTTRTC-3' DKGVVPLA
G6PD sense 5'-CATAATGGGIGCIWSIGGNGAYYT-3' IMGASGDL
  sense 5'-GTCAGCTACTGGNGAYYTNGCNAA-3' ATGDLAK
  sense 5'-GTCATCTTCGGIGCNWSNGGNGAY-3' GASGD
  sense 5'-GACCACTAYYTNGGNAARGARATG-3' DHYLGKEM
  antisense 5'-AGATGCAGGYTTYTCCATNGCNAC-3' VAMEKPAS
  antisense 5'-GTCGSWNCKNACRAARTGCATYTG-3' QMHFVRSD
GAG sense 5'-CAGTGGCGGIMGIGARCAYRC-3' SGGREH(A/T)
  sense 5'-GATGCCNCCIGCNCARGAYC-3' MPPAQDH
  sense 5'-GAGTTYAAYTGYMGNTTYGG-3' EFNCRFG
  antisense 5'-CCGAANCKRCARTTRAAYTC-3' EFNCRFG
  antisense 5'-GTTRAAGGTNCKIGYCATYTC-3' EM(A/T)RTFN
  antisense 5'-GAAGCCTGCIARRTCRTAYTC-3' EYDLAGF
  antisense 5'-GTCRTTIACRCACATNGCNAC-3' VAMCVND
PGK sense 5'-CATCCGIGTNGAYTTYAAYGTNCC-3' RVDFNVP
  antisense 5'-GAAGACACCNGGNGGNCCRTTCCA-3' WNGPPGVF
  antisense 5'-CTTGTCISWIACYTTNGCNCCNCC-3' GGAKVSDK
  antisense 5'-GTTCTCDATNARYTGDATYTTRTC-3' DKIQLI
TPI sense 5'-CCGGTACCAAYTGGAARATGAAYGG-3' NWKMNG
  antisense 5'-ATGGATCCCCIACIARRAAICCRTC-3' DGFLV
  antisense 5'-GCCTATGGCCCANACNGGYTCRTA-3' YEPVWAIG
  1. EcoRI, BamHI and KpnI restriction sites are underlined. Amino acid sequence used for designing each degenerate primer is also shown. Abbreviation for each protein name: ALDa, fructose-bisphosphate aldolase A; ALDb, fructose-bisphosphate aldolase B; ALDc, fructose-bisphosphate aldolase C; G6PD, glucose-6-phosphate 1-dehydrogenase; GAG, a trifunctional protein with glycinamide ribonucleotide synthetase (GARS)-aminoimidazole ribonucleotide synthetase (AIRS)-glycinamide ribonucleotide formyltransferase (GART); PGK, phosphoglycerate kinase; TPI, triosephosphate isomerase.
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