Skip to main content

Table 4 Degenerate primers used for the cloning of cDNAs

From: Basal jawed vertebrate phylogeny inferred from multiple nuclear DNA-coded genes

Target

 

Primer

Amino acid sequence

ALDa-c

sense

5'-CAGGATCCAARGGIATHYTIGCNGC-3'

GKGILAA

 

sense

5'-GGCCGTCGTCGGNATHAARGTNGA-3'

VGIKVD

 

antisense

5'-CAGAATTCGTIACCATRTTIGGYTT-3'

KPNMVT

 

antisense

5'-GTGIACGCAICKCCAYTTNGCRAA-3'

FAKWRCV

ALDc

antisense

5'-CTCYTTYTTNCCNCCCCANGCYTT-3'

KAWGGKKE

ALDa,c

antisense

5'-GGCTAGNGGNACNACNCCYTTRTC-3'

DKGVVPLA

G6PD

sense

5'-CATAATGGGIGCIWSIGGNGAYYT-3'

IMGASGDL

 

sense

5'-GTCAGCTACTGGNGAYYTNGCNAA-3'

ATGDLAK

 

sense

5'-GTCATCTTCGGIGCNWSNGGNGAY-3'

GASGD

 

sense

5'-GACCACTAYYTNGGNAARGARATG-3'

DHYLGKEM

 

antisense

5'-AGATGCAGGYTTYTCCATNGCNAC-3'

VAMEKPAS

 

antisense

5'-GTCGSWNCKNACRAARTGCATYTG-3'

QMHFVRSD

GAG

sense

5'-CAGTGGCGGIMGIGARCAYRC-3'

SGGREH(A/T)

 

sense

5'-GATGCCNCCIGCNCARGAYC-3'

MPPAQDH

 

sense

5'-GAGTTYAAYTGYMGNTTYGG-3'

EFNCRFG

 

antisense

5'-CCGAANCKRCARTTRAAYTC-3'

EFNCRFG

 

antisense

5'-GTTRAAGGTNCKIGYCATYTC-3'

EM(A/T)RTFN

 

antisense

5'-GAAGCCTGCIARRTCRTAYTC-3'

EYDLAGF

 

antisense

5'-GTCRTTIACRCACATNGCNAC-3'

VAMCVND

PGK

sense

5'-CATCCGIGTNGAYTTYAAYGTNCC-3'

RVDFNVP

 

antisense

5'-GAAGACACCNGGNGGNCCRTTCCA-3'

WNGPPGVF

 

antisense

5'-CTTGTCISWIACYTTNGCNCCNCC-3'

GGAKVSDK

 

antisense

5'-GTTCTCDATNARYTGDATYTTRTC-3'

DKIQLI

TPI

sense

5'-CCGGTACCAAYTGGAARATGAAYGG-3'

NWKMNG

 

antisense

5'-ATGGATCCCCIACIARRAAICCRTC-3'

DGFLV

 

antisense

5'-GCCTATGGCCCANACNGGYTCRTA-3'

YEPVWAIG

  1. EcoRI, BamHI and KpnI restriction sites are underlined. Amino acid sequence used for designing each degenerate primer is also shown. Abbreviation for each protein name: ALDa, fructose-bisphosphate aldolase A; ALDb, fructose-bisphosphate aldolase B; ALDc, fructose-bisphosphate aldolase C; G6PD, glucose-6-phosphate 1-dehydrogenase; GAG, a trifunctional protein with glycinamide ribonucleotide synthetase (GARS)-aminoimidazole ribonucleotide synthetase (AIRS)-glycinamide ribonucleotide formyltransferase (GART); PGK, phosphoglycerate kinase; TPI, triosephosphate isomerase.