Table 1 Primers used to amplify the p27kip1 gene for single-strand conformation analysis (SSCA).
Amplified sequence | Primer sequence* | Annealing temperature | PCR product length (bp) |
|---|---|---|---|
promoter | P5-F: GGAGGAAGGAAGGAGCTGTTGTA P5-R: AAGCCAAAGCGAACGTCTTTC | 62°C | 360 |
promoter | P4-F: ATGATAAGTGCCGCGTCTACTCCT P4-F: ACCAGAGGACCGCGAAGGT | 62°C | 310 |
promoter | P3-F: GGCCGAGCTGGGGGCAGCT P3-R: TTAACCTGGCCCCGGCGC | 66°C | 362 |
promoter | P2-F: TCGGGGAGGCGGCGCGCTCG P2-R: AGAGGGTGGCAAAGCCCGTC | 62°C | 360 |
promoter | P1-F: TGGGTTCGCGGGACCGCG P1-R: CTGCCTGGCGTCCATCCG | 62°C | 385 |
Exon 1 | E1-F: TGCGAGTGTCTAACGGGAGC E1-R: TTACCGTCGGTTGCAGGTC | 58°C | 472 |
Exon 2 | E2-F: TAAAGATTGTGTGTTCTTTTTAA E2-R: TATCGTGAGGTCTGAAGGCC | 55°C | 230 |