SNP | Primer sequence* | Annealing temperature (Enzyme) | PCR product length after digestion (bp) |
---|---|---|---|
-838C>A | F: TCCAGGTCCCGGCTTCCCGGt** R: CCTGCTCTGGCTGGCCTCGGAG | 65°C (TaqI) | 177 (A allele) 153+24 (C allele) |
-79C>T | F: TGATCAGCGGAGACTCGGCG R:CTGCCTGGCGTCCATCCG | 58°C (HaeIII) | 275 (T allele) 140+135 (C allele) |
+326T>G (V109G) | F: TGCGAGTGTCTAACGGGAGC R: TTACCGTCGGTTGCAGGTC | 58°C (BglI) | 472 (T allele) 316+156 (G allele) |