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Table 2 Primers used to genotype the single-nucleotide polymorphisms (SNPs).

From: A single-nucleotide polymorphism in the human p27kip1gene (-838C>A) affects basal promoter activity and the risk of myocardial infarction

SNP

Primer sequence*

Annealing temperature (Enzyme)

PCR product length after digestion (bp)

-838C>A

F: TCCAGGTCCCGGCTTCCCGGt**

R: CCTGCTCTGGCTGGCCTCGGAG

65°C (TaqI)

177 (A allele) 153+24 (C allele)

-79C>T

F: TGATCAGCGGAGACTCGGCG

R:CTGCCTGGCGTCCATCCG

58°C (HaeIII)

275 (T allele) 140+135 (C allele)

+326T>G (V109G)

F: TGCGAGTGTCTAACGGGAGC

R: TTACCGTCGGTTGCAGGTC

58°C (BglI)

472 (T allele) 316+156 (G allele)

  1. * F: forward; R, reverse. ** A mismatch to create a TaqI site when -838C is present is shown in lower case.