Figure 1

DLG, GluRIIA, and GluRIIB are localized postsynaptically at the Drosophila NMJ A: Confocal projection of two boutons in a Drosophila third instar neuromuscular junction, visualized using the neuronal membrane marker anti-HRP (red) and anti-DLG antibodies (green). Scale bar = 2 μm. B: Isosurface projection generated from the confocal stack shown projected in A. At this stage of development, larval boutons are partially embedded in postsynaptic muscle membrane. DLG immunoreactivity surrounds the boutons, consistent with postsynaptic localization. C, D: Confocal projections of larval NMJs visualized using antibodies that recognize DLG (green) and the glutamate receptor subunits GluRIIA or GluRIIB (magenta, in panels C and D, respectively). Note the incomplete overlap of DLG and glutamate receptors; glutamate receptor immunoreactivity falls within the area stained by DLG, but not all DLG immunoreactivity overlaps with glutamate receptor immunoreactivity. Scale bar = 10 μm.