Figure 5 From: Microtubule plus-ends reveal essential links between intracellular polarization and localized modulation of endocytosis during division-plane establishment in plant cells PM targeted EMT plus ends probe the areas occupied by the preceding PPB and align the cell plates for proper docking at the parental walls. Green: GFP-MAP4 (in A, F-I), GFP-AtEB1 (in B-E, J-O and P). Red: FM4-64 (in A, F-O), YFP-MAP4 (in P). (A) Discontinuity of the vacuolar structures in the preceding PPB site (arrowheads) is maintained at the spindle stage, as visualized with FM4-64 labeled vacuoles and GFP-MAP4 labeled microtubules.(B-C) At the onset of the phragmoplast stage, GFP-AtEB1 labeled EMT plus ends (red arrowheads) originating from the former spindle poles grow towards the cortex (see additional file 9: Movie 9). Occasionally, they grow towards the polar areas (yellow arrowhead). (D) GFP-AtEB1 labeled EMT plus ends (arrowheads) are attracted to the cortical areas marked by the preceding PPB. At late telophase, the distance through which GFP-AtEB1 labeled EMT plus ends reach towards the cortex is reduced. (E) 3-D projection showing GFP-AtEB1 labeled EMT plus end trajectories directed towards the cortex, which are different from the main phragmoplast structure. GFP-MAP4 labeled EMTs (F-I) or GFP-AtEB1 labeled EMT plus ends (J-M) continue to reach the cortex at the former PPB site and display close proximity to FM4-64 labeled endosomes (red arrow and arrowheads). These endosomes display movement towards the minus end of these EMTs. (N-O) GFP-AtEB1 labeled plus end growth of EMTs (arrowheads) towards opposite sides of the cortex is maintained during cell plate and phragmoplast tilting (see additional file 10: Movie 10). (P) Enrichment of GFP-AtEB1 labeled microtubule plus ends (arrowhead) but not of YFP-MAP4 labeled microtubular parts at the phragmoplast midline. Time in F-I is given in seconds while that in J-O is indicated in minutes. Bars in A-O represent 8 μm while that in P represents 10 μm.Back to article page