Raptor proteins in eukaryotes are highly conserved. (A) Similarity plot of Raptor homologues from the vascular plants Arabidopsis, Medicago truncatula and Oryza sativa, the fungus S. pombe (Mip1p), and mammals. The X-axis represents residue number; the Y-axis represents percent identity at that residue from 0% (0) to 100% (1). (B) Schematic diagram showing the position of the Raptor N-terminal Conserved / putative Caspase domain (RNC/C) region, HEAT repeats (H), and WD-40 repeats (WDx7) common to all Raptor proteins. (C) Phylogeny of plant, animal and fungal Raptor proteins. Bootstrap values, calculated using both parsimony (left) and maximum likelihood (right) are shown to the left of the clades they describe. The two Arabidopsis Raptor proteins, AtRaptor1A and AtRaptor1B, resolve as a single clade with 100% confidence. The alignment was generated using Megalign (DNAStar), the similarity plot was generated from this alignment using VectorNTI, and bootstrap values were calculated using PAUP*4.0b.