AtRaptor loci and insertion allele characterization. (A) AtRaptor1A and AtRaptor1B loci. Genomic sequence is depicted as a thin central line. Thick blocks indicate exons. Coding exons span the central line; exons encoding untranslated regions are fully below the central line. The positions of the T-DNA insertions are depicted with inverted triangles. (B) Reverse-Transcribed RNA-template Polymerase Chain Reactions (RT-PCR) on plants homozygous for both wild-type AtRaptor alleles (Col), the AtRaptor1A insertion allele (A-) or the AtRaptor1B insertion allele (B-), using primers spanning the AtRaptor1A insertion site, the AtRaptor1B insertion site, or control primers. Both AtRaptor insertion alleles abolish accumulation of the wild-type transcript from their locus.