Figure 3

Seedling root phenotype of AtRaptor1B -/- (B-) mutants. (A), (B). Col and B- seedlings on growth medium, four days after germination. The B- root has not penetrated the medium and is thick, hairy and coiled. (C) Col, A- and B- seedlings at 8 days after germination in light. Scale bar is in mm. (D) Same genotypes and age as C, grown in the dark. (E) Measurements of populations grown as in C, D. Root length is indicated in tan; shoot length is dark green. (F) B- and Col seedlings grown on vertical plates for 12 days, and then returned to horizontal growth for three days. B- roots are thin, straight and hairless on vertical plates (compare to 3B), and revert to coiled growth only in tissue generated after being placed horizontally. (G) Quantification of results in (F). B- seedlings grown on vertical plates are intermediate in length between flat-grown B- seedlings and Col seedlings. (H), (I) Col and B- root tips, viewed under bright field microscopy. Scale bar = 100 μm. B- root tips contain all the cell types seen in Col root tips, but the overall morphology is blunt and rounded compared to Col.